perilipin antibody  (Bio-Techne corporation)

Journal: Bone Research

Article Title: A novel multifunctional radioprotective strategy using P7C3 as a countermeasure against ionizing radiation-induced bone loss

doi: 10.1038/s41413-023-00273-w

Figure Lengend Snippet: P7C3 treatment (20 mg·kg −1 ) reduces bone marrow adiposity and cell senescence following exposure to harmful irradiation ( n = 6). a Representative photomicrographs of Sudan Black B staining for adipocytes and lipids (stained black). The nuclei were counterstained with nuclear fast red (pink). b Quantification of Sudan Black B + cell number in each of the groups. c Quantification of Sudan Black B + cellular area and d cell diameter. e Representative photomicrographs of perilipin immunohistochemical staining. Red arrows indicate perilipin + cells. f Quantification of perilipin + cells. g Perilipin + cellular area. h Perilipin + cell diameter. i Representative micrographs of Oil Red O staining of the rat bone sections. P7C3 reduced IR-induced bone marrow adiposity. j Quantification of Oil Red O staining. k Representative photomicrographs of SA-β-Gal staining for senescent cells (stained blue). l Quantitative analysis of SA-β-Gal + cell intensity. m Representative images of senescence-associated secretory phenotype (SASP) TNF-α + cell staining. Red arrows indicate TNF-α + cells. n Quantification of TNF-α + cells in each of the groups. **** P < 0.000 1. o Representative images of p21 + cell staining. p Quantification of p21 + cells in each of the groups. **** P < 0.000 1. q Representative images of p16 + cell staining. r Quantification of p16 + cells in each of the groups. **** P < 0.000 1

Article Snippet: The following antibodies were used as primary antibodies overnight incubation: RANKL (NB100-56512, Novus Biologicals, USA) and perilipin (NB110-40760, Novus Biologicals, USA).

Techniques: Irradiation, Staining, Immunohistochemical staining